Direct Visualization of Fis-DNA Interactions

Direct Visualization of Single-Molecule DNA-Binding Proteins Along DNA to Understand DNA–Protein Interactions

Direct visualization of transient thermal response of a DNA origami.

The DNA origami approach enables the construction of complex objects from DNA strands. A fundamental understanding of the kinetics and thermodynamics of DNA origami assembly is extremely important for building large DNA structures with multifunctionality. Here both experimental and theoretical studies of DNA origami melting were carried out in order to reveal the reversible association/disassoc...

Direct visualization of a DNA glycosylase searching for damage.

DNA glycosylases preserve the integrity of genetic information by recognizing damaged bases in the genome and catalyzing their excision. It is unknown how DNA glycosylases locate covalently modified bases hidden in the DNA helix amongst vast numbers of normal bases. Here we employ atomic-force microscopy (AFM) with carbon nanotube probes to image search intermediates of human 8-oxoguanine DNA g...

Direct inference of protein-DNA interactions using compressed sensing methods.

Compressed sensing has revolutionized signal acquisition, by enabling complex signals to be measured with remarkable fidelity using a small number of so-called incoherent sensors. We show that molecular interactions, e.g., protein-DNA interactions, can be analyzed in a directly analogous manner and with similarly remarkable results. Specifically, mesoscopic molecular interactions act as incoher...

Direct Visualization of Quadrics

Today, most of the powerful graphic systems are based on 3D-triangle display methods. However, this approach generates well-known problems, like the low quality of contours and shading, and the necessity to have large amounts of primitives to display complex scenes. A way to solve these problems is to use higher level primitives, among which a very interesting one is the quadric surface. We stu...